Coding

Part:BBa_K4644024

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-10-10)


DnaK

        DnaK is a chaperone protein that assists with polypeptide folding.In conjuction with DnaJ, DnaK assists in correct polypeptide folding and is the Prokaryotic homologue of the Eukaryotic chaperone system 'Hsp70'. This gene, along with DnaJ, GrpE, GroEL and GroES forms the heat shock system in bacteria.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 727
    Illegal BglII site found at 930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 156
    Illegal NgoMIV site found at 1671
    Illegal AgeI site found at 399
    Illegal AgeI site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None