Coding
Part:BBa_K4644024
Designed by: Han Cao Group: iGEM23_BUCT (2023-10-10)
DnaK
DnaK is a chaperone protein that assists with polypeptide folding.In conjuction with DnaJ, DnaK assists in correct polypeptide folding and is the Prokaryotic homologue of the Eukaryotic chaperone system 'Hsp70'. This gene, along with DnaJ, GrpE, GroEL and GroES forms the heat shock system in bacteria.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 727
Illegal PstI site found at 860
Illegal PstI site found at 970
Illegal PstI site found at 1321 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 727
Illegal PstI site found at 860
Illegal PstI site found at 970
Illegal PstI site found at 1321 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 727
Illegal BglII site found at 930 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 727
Illegal PstI site found at 860
Illegal PstI site found at 970
Illegal PstI site found at 1321 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 727
Illegal PstI site found at 860
Illegal PstI site found at 970
Illegal PstI site found at 1321
Illegal NgoMIV site found at 156
Illegal NgoMIV site found at 1671
Illegal AgeI site found at 399
Illegal AgeI site found at 871 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |